New research methods in GBUZ RM "MRCB"

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In the clinical diagnostic laboratory introduced 44 new forms of research.

By ELISA 25 studies:

Tumor markers:

1. Neuron specific enolase serum (NSE) – diagnosis and monitoring of treatment of small cell lung cancer; neuroblastoma.

2. Fragment of cytokeratin 19 (CYFRA-21-1) – diagnosis and control of treatment of non-small cell lung carcinoma, squamous cell carcinoma and lung adenocarcinoma, invasive carcinoma of the bladder.

3. HE-4 for the diagnosis, prognosis and monitoring of treatment of cancer of the ovary and endometrium.

4. The tumor marker CA 242 in serum for the diagnosis and evaluation of the effectiveness of treatment of cancer of the pancreas, colon and rectum.

5. UBC (urinary bladder cancer Antigen) is a urine test.

Diagnostica, diseases of the gastrointestinal tract:

1. Determination of pancreatic elastase in stool – the "gold standard" diagnosis of diseases of the pancreas.
2. The determination of calprotectin in stool – diagnosis of the degree of activity of inflammatory process in the intestine, Crohn's disease.
3. ELISA test for diagnosis of autoimmune diseases
4. Detection of antibodies to SS-A antigen in blood serum (Sjogren's Syndrome, systemic lupus erythematosus)
5. Determination of antibodies to myeloperoxidase (MPO) microscopic arteritis, nadasny polyarteritis, Churg Strauss.
6. Detection of antibodies to LKM-1 (diagnosis of autoimmune hepatitis)
7. Detection of specific immunoglobulin G to diagnose Crohn's disease.

Vitamins:

• Determination of vitamin b 12.
• Determination of folate in erythrocytes.
• Determination of vitamin D.
• Determination of vitamin B1 (thiamine).
• Determination of vitamin B2 (Riboflavin).
• Definition of folic acid.</li>
• Definition of Pantothenic acid.
• Determination of vitamin B12.
• Definition of Niacin.
• Definition of Biotin.
• Determination of water soluble vitamin C.
• Determination of retinol-binding protein.
• Determination of vitamin D-binding protein.
• Determination of vitamin B6 (pyridoxine).

Diagnosis of autoimmune diseases:

1. Detection of antibodies to SS-A antigen in blood serum (Sjogren's Syndrome, systemic lupus erythematosus)
2. Determination of antibodies to myeloperoxidase (MPO) microscopic arteritis, nadasny polyarteritis, Churg Strauss.
3. Detection of antibodies to LKM-1 (diagnosis of autoimmune hepatitis)
4. Detection of specific immunoglobulin G to diagnose Crohn's disease.

PCR 14 studies:

1. the group of mutations in the genes.

1. Prothrombin or factor II, factor VII, factor V or is it laganovska mutation, fibrinogen;
2. Definition of hereditary predisposition to breast cancer and ovarian;
3. A study on the determination of gene polymorphism for interleukin 28 patients with hepatitis C receiving antiviral therapy;
4. Determination of gene polymorphisms of the lactase (LPH))
5. Determination of gene polymorphisms responsible for individual sensitivity to warfarin is used to evaluate the effectiveness of the application and dose adjustment of warfarin

Group 2 - studies for the detection of molecules of RNA or DNA of pathogens (viruses or bacteria) in a biological material –

1. Diagnosis of human papillomavirus,
2. Diagnostics of viral hepatitis D and G, studies on the genotyping of hepatitis C
3. Issledovanie biological material of the urogenital tract in dysbiosis (identifying the etiological structure disease), enabling the identification of pathogenic microorganisms in the 24-48 hours is an alternative bacteriological research.

Hemostasis system 5 research:

The study of the hemostatic system in thrombophilia:

• Determination of the activity of von Willebrand factor
• Determination of activity of factor VIII (antihemophilic globulin)
• Determine the activity of factor VII
• Protein With
• Protein S

The study of antibiotic resistance:

• PCR study of the genes of antibiotic resistance.
• The system of control of antibiotic resistance in the hospital.

Determination of composition of kidney stones.

In rontgendiagnostik:

CT determination of coronary calcium coronary syndrome without changes on ECG screening.

CT coronary angiography with the construction of a 3D image - screening asymptomatic persons with low or moderate risk factors for coronary heart disease.

• Cardialgia with negative or questionable load tests.
• Atypical chest pain in patients after angioplasty of the coronary arteries, the patency of coronary bypass grafts, stents.
• Diagnosis of aneurysms and congenital anomalies of the coronary arteries.

The main tasks of non-invasive coronary angiography:

• detection of coronary stenoses;
• evaluation of stent patency and functioning of the shunts;
• the need to focus on invasive CAG.

The advantages of cardiac CT:

• the possibility of screening for coronary atherosclerosis and ischemic heart disease;
• non-invasive (contrast medicine is injected into the cubital vein as in standard CT examinations);
• a highly informative andresearch, early detection, as the pathological process of vessels, and its causes
• the method is standardized, easily tolerated by patients, its data will be well documented.

Cardiac CT is not shown:

• intractable heart rate correction;
• the defeat of the distal branches of the coronary arteries;
• contraindications for injection of contrast agents:

- allergic reaction to iodine-containing contrast agent;

- increased creatinine of blood plasma above the norm;

- hyperthyroidism;

• the inability of the patient to hold their breath, the inappropriate behavior;
• severe degree of calcification (more than 600).

MSCT coronary angiography is the most informative in case of doubtful clinical data allowing to exclude coronarienne the cause of thoracalgia.

The inclusion of MSCT-coronary calcium index (CaScoring) in the algorithm of examination of patients with suspected coronary artery disease will help to identify patients with coronary artery disease from the group of practically healthy people, to determine the risk of sudden coronary events

Multislice computed tomography due to the large bandwidth has become a universal method of screening for coronary artery disease

Magnetic resonance enterography, or Mr enterography , is a minimally invasive diagnostic test used to visualize internal structure of the small intestine. Through this study we can identify areas of inflammation (swelling and irritation), bleeding, and other diseases of the small intestine.

Indications for the behavior of Mr-enterography:

• Erosive-ulcerative lesions of the gut identified in other departments.
• Suspected isolated lesions of the distal small intestine.
• Persistent pain in stomach with the exception of pathology in other organs.
• The signs of malignant intoxication or metastases.
• Suspected small bowel obstruction, as the study clearly shows the area of blockage of the lumen of the intestine.
• The exception of anomalies of the small intestine that threaten the patient's life.

• Neoplasms of the small intestine;
• Crohn's Disease
• Identify the areas of irritation and swelling
• Definition of abscesses filled with purulent pockets in the intestine walls
• Identify any types of intestinal obstruction
• Evaluation of the effectiveness of the treatment

Mr - mammography with contrast and graphs of accumulation of contrast - differential diagnosis of benign and malignant lesions by determining the prevalence of the process and the involvement of l/y.

Mr-urography with contrast and definition of renal excretory function, with the construction of a 3D image space-occupying lesions of the kidneys, stricture of the ureter, R-negative stones, allergic reaction to iodine containing contrast preparation.

MRI of renal vessels and abdominal aorta with a/V enhancement - pathology of renal vessels (stenosis, aneurysm)

Mr of the temporomandibular joints - the differentiation of TMJ

In ultrasound and functional diagnostics:

Diagnosis of disorders of breathing during sleep.

The stress ECHO.

Transesophageal and endocardial EX.